DOI: 10.21276/ajptr
Mon, 22 Apr 2019

Evaluation of Matrix Effect in Bio Estimation of Prasugrel Hydrochloride by Liquid Chromatography-Mass Spectrometry

Muruganathan Gandhimathi1*, Krishna Kiran Gupta1, Thengungal Kochupappy Ravi1

1.Sri Ramakrishna Institute of paramedical Sciences, Coimbatore- 641 044, Tamilnadu, India


Liquid chromatography-mass spectrometry (LC-MS) is sensitive technique that is affected by matrix effect. It arises due to phospholipids, proteins or metabolites present in biological fluids. It either increases or decreases the ionization of sample and affects detection limits. By careful evaluation of matrix effect on the drugs and their ionization in LC-MS the sensitivity could be improved. Evaluation of matrix effect for bio estimation of prasugrel hydrochloride is done by sample preparation methods, chromatographic conditions, internal standard and mass spectrometric conditions. A LC-MS method was developed for prasugrel hydrochloride with mobile phase consisted of ammonium formate (5mM, pH 7.5): acetonitrile (30:70, %v/v) at 1 mL/min flow rate and PDA detection at 220 nm. The mass detection method was after ionization with ESI probe, CDL temperature 230°C, detector voltage-1.5 Kv, nebulisation gas flow-1.5 L/min and column temperature was 30.5°C. The molecular ion peak was identified at m/z of 410 and product ion peak at m/z 374. The plasma spiked with prasugrel was subjected to each evaluation process and matrix effect was studied.  In sample preparation technique, the extraction efficiency and process efficiency were also calculated. The study results have shown that the matrix effect could be reduced systematically by the procedures performed and the sensitivity of LC-MS in the detection and quantification was increased to detect 20 ng/mL of Prasugrel from plasma.

Keywords: Matrix effect, Prasugrel, LC/MS, biological fluid

[PDF]   Viewed: 1326   Downloaded: 141
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter