DOI: 10.21276/ajptr
Wed, 16 Jan 2019

A Newer RP - HPLC Method for the Estimation of Dapsone in Bulk and In Pharmaceutical Formulations

M.Madhu1, V.Sreeram2,  A.V.D.Nagendrakumar3 , Prof.T.V.Reddy4

1. Department of chemistry, P.B. Siddhartha College of Arts & Science, Vijayawada, Krishna (Dt) -520010.A.P.INDIA

2. Department of chemistry, A.G.& S.G. Siddhartha College of Arts & Science, Vuyyuru, Krishna (Dt) -521165.A.P. INDIA.

3. Department of Chemistry, GITAM University, Visakhapatnam-530 045, Andhra Pradesh, India

4. Department of H & S, Malla Reddy college of Engineering, Secunderabad., India


A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method is developed and validated for the estimation of Dapsone in tablet dosage form. The expected separation and peak shapes were obtained on Luna C18, 15 cm x 4.6 mm (5 μm)  column. To have an ideal separation of the drug under isocratic conditions, mixtures of solvents like methanol and water with or without different buffers indifferent combinations were tested as mobile phases on a Luna C18, 15 cm x 4.6 mm (5 μm) column. A mixture of Methanol:Water in the ratio of 40:60 v/v was proved to be the most suitable of all the combinations since the chromatographic peak obtained was better defined and resolved and almost free from tailing.  The flow rate was 1.0ml/min and effluents were monitored at 260 nm. The retention time for Dapsone was ± 2.4 min. The method was validated for accurate, precise, simple, sensitive and rapid and can be applied successfully for the estimation of Dapsone in bulk and in pharmaceutical formulations without interference and with good sensitivity. And recovery of Dapsone from tablet formulation was found to be 93%. The proposed method was successfully applied for the quantitative determination of Dapsone in tablet formulation.

Keywords: Dapsone, HPLC, Linearity, Validation, 260 nm

[PDF]   Viewed: 1924   Downloaded: 239
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter