DOI: 10.21276/ajptr
Thu, 24 Jan 2019

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Losartan Potassium and Perindopril In Tablet Dosage Form

Naik Desai Prabhat Prabhaker1*, M. G. Pai2

1.Department of Chemistry, DnyanprassarakMandal’s College and Research Centre, Mapusa Goa,

2. Department of Pharmaceutical Analysis, Goa College of Pharmacy, Panaji Goa.


The present work describes a new simple, sensitive and precise reverse phase high performance liquid chromatographic method (RP-HPLC) for the simultaneous estimation of Losartan potassium (LP) and Perindopril erbumine (PE) in bulk and in pharmaceutical dosage forms. Chromatographic separation was performed on KNAUER High Performance Liquid Chromatographic System with C18 column of Make: Thermo Hypersil – ODS of dimensions 250 x 4.6mmwith a mobile phase comprising of 0.01M potassium phosphate buffer (pH 3.5): Acetonitrile: Methanol in the ratio of 5:55:40v/v. the pH of buffer was adjusted with ortho phosphoric acid. The flow rate was 1.0 ml/min with detection with detection at 210nm.As per International Conference on Harmonization (ICH) guidelines the method was validated for linearity, precision, limit of quantitation, limit if detection and robustness. Linearity of LP was found to be in the range of 350µg/ml-650µg/ml and 28µg/ml-52µg/ml for PE. The correlation coefficient for LP and PE was found to be 0.997 and 0.998 respectively. The mean recoveries obtained for LP and PE was found to be 100.222% and 99.844% respectively. The developed analytical method was found to be accurate, linear, specific, and precise which is evident from the statistical data.

Keywords: Losartan Potassium, Perindopril Erbumine, RP-HPLC, simultaneous estimation, validation.

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