DOI: 10.21276/ajptr
Thu, 24 Jan 2019

Development and validation of a method for densitometric analysis of umbelliferone, β-sitosterol and methoxsalen in polyherbal formulation

Deepa Iyer 1*, U. K. Patil 2

1. LNCT College of Pharmacy, Kalchuri Nagar, Raisen Road, Bhopal (462021), M.P., India;

2. Dr. H.S. Gour University, Sagar - 470003, (M.P.) India


Herbal medicines are generally available as a mixture of more than one plant constituent. It is important to quantify the maximum possible number of markers in such herbal formulations by which the quality of the formulations may be assessed. TLC densitometric methods were developed using High performance thin layer chromatography for the quantification of the three marker compounds, umbelliferone, β-sitosterol and methoxsalen, from the polyherbal tablet formulation. Solvent systems were optimized to achieve best resolution of the marker compounds from the other components of the sample.  From the various solvent systems tried, the one containing toluene: ethyl acetate: methanol: formic acid (3: 3: 0.2: 0.8) gave best resolution of umbelliferone (Rf=0.35), β-sitosterol (Rf=0.47) and methoxsalen (Rf=0.6) in the presence of the other compounds in the polyherbal tablet sample and enabled the quantification of the marker compounds. The methods were validated in terms of precision, repeatability and accuracy. The method was found to be suitable for the quantification of these marker compounds in polyherbal formulation.

Keywords: densitometric, umbelliferone, β-sitosterol and methoxsalen.

[PDF]   Viewed: 588   Downloaded: 70
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter