DOI: 10.21276/ajptr
Wed, 16 Jan 2019

New Validated HPLC Method for the Estimation of Oxazepam In Pharmaceutical Formulation

V.Sreeram1*, K.Satyanarayana2, A.V.D.Nagendra kumar3

1.Department of chemistry(P.G), A.G.& S.G. Siddhartha Degree College of Arts & Science, Vuyyuru, Krishna (Dt) -521165.A.P.INDIA.

2. Department of chemistry,(U.G), A.G. & S.G. Siddhartha Degree College of Arts & Science, Vuyyuru, Krishna (Dt) -521165.A.P.INDIA.

3. Department of Chemistry, GITAM University, Visakhapatnam-530 045, Andhra Pradesh, India


A simple, selective, linear, precise and accurate HPLC method was developed and validated for rapid assay of Oxazepam in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.0 ml/min was employed.  The chromatographic analysis was performed on a ODS 5 μm (4.6 mm x 15 cm) or equivalent column at ambient temperature. The mobile phase consisted of Methanol: Water in the ratio of 65:35v/v. The UV detection wavelength was 230nm and 100µl sample was injected. Flow rate was found to be 1.0.  The retention time for Oxazepam was identified. The Average percentage recovery of the method was in the range of 0.5.  The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation.

Keywords: Oxazepam, HPLC, UV detection, Recovery, Precise.

[PDF]   Viewed: 535   Downloaded: 81
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter