DOI: 10.21276/ajptr
Mon, 16 Jul 2018

Analytical Method Development and Validation for Enrofloxacin in Bulk and Formulation by RP-HPLC Method

Kandarp M. Patel1, B.N. Suhagia*2, Indrajeet Singhvi3

1..Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.

2. Dean, Faculty of Pharmacy, Dharmsinh Desai University, Nadiad-387001, Gujarat, India.

3. Dean, Faculty of Pharmacy, Pacific University, Udaipur, Rajasthan, India.


ABSTRACT

Enrofloxacin (EFX) is a third generation Fluoroquinolone with a broad spectrum antibacterial activity. Enrofloxacin hydrochloride is 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid. A Sensitive, simple and rapid reverse phase high performance liquid chromatographic method was developed for the determination of Enrofloxacin (EFX) in tablet dosage form. The chromatographic separation was performed on a Kromasil C-18 column (250mm x 4.6 mm x 5µ) in isocratic mode using phosphate buffer pH 3:Methanol (40:60 v/v), pH adjusted to 3.0 using orthophosphoric acid as mobile phase at a flow rate of 1.0 ml/min with column temperature 30 OC. The quantification was performed at 280 nm. The method showed good linearity over the concentration range of 5-25 µg/ml with correlation coefficient r2 0.9996. LOD and LOQ was found to be 1.0 and 3.0. The developed RP HPLC method was applied to EFX in tablet dosage form and results were found to be in agreement with the label claim.

Keywords: Enrofloxacin, RP-HPLC, Fluoroquinolone, ICH guideline (Q2R1)


[PDF]   Viewed: 192   Downloaded: 45 DOI No: 10.21276/ajptr.2018.08.02.13
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter