In the present communication, finger print of two ethano-botanically important Anisomeles species has been developed. A sensitive and reliable densitometric High Performance Thin Layer Chromatography (HPTLC) method has been developed for the quantification of quercetin, β-sitosterol, stigmasterol, catechin and ovatodiolide present in Anisomeles indica and Anisomeles malabarica.  Chromatographic analysis was performed using methanol, chloroform, acetone and ethanol extract of these plants were developed in the different solvents such as toluene, chloroform, ethyl acetate, methanol at various proportions. Detection and quantification of all phytoconstituents was done by densitometric scanning at different wavelengths. These finger prints would be helpful in the standardization of these species.   Key words: HPTLC, Anisomeles indica, Anisomeles malabarica, phytoconstituents, standardization