Beta-blockers are the first line drugs in lowering the elevated and normal intraocular pressure associated with neuropathy of glaucoma, but for many reasons the use of this group of drugs requires supportive medications. The systemic side effects due to overdose of these drugs leads to pathetic complications in patients suffering with cardiac and pulmonary diseases. This article provides necessary information for the safe use of beta-blockers with detailed information about the disease glaucoma. Recent advances in the glaucoma therapy with beta-blockers and novel drug delivery systems developed are reviewed in this study.   Key words: Glaucoma, beta-blockers, intraocular pressure, Timolol, Levobunolol