A simple, sensitive and accurate spectrophotometric method was developed in ultraviolet region for the estimation of Glibenclamide in pure drug, pharmaceutical formulation. Linear response obtained was in the concentration range of 5-30µg/ml with correlation coefficient of 0.999 in acetronitrile: 0.2M NaOH (20:80). Excellent recovery proved that the method was sufficiently accurate. There is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated by recovery studies according to ICH Q2B guidelines. Key words: Glibenclamide, UV- Spectrophotometry, recovery, accuracy.