The plant “Jussiaea hyssopifolia” has great medicinal value and reported as astringent, carminative, laxative, diuretic and anthelmintic properties. In the present investigation, different parameters are applied for the physico-chemical studies include evaluation of colour, consistency of different extracts, extractive value, ash value, moisture content, fluorescence analysis and also qualitative phytochemial screening was performed. Thus, present study revealed the plant extract contain different chemical constituents like alkaloid, phenolic compound, saponin, flavonoid, proteins & amino acids. Phytoconstituents in different extracts could be useful in setting some diagnostic indices for the identification and preparation of plant monographs. Hence objective of present investigation was planned to find out the quality level of different extract & powder drug of Jussiaea hyssopifolia in physicochemical studies. Key Words: Jussiaea hyssopifolia, Powdered drug & Extract, Phytochemial screening, Fluorescence analysis.