The present study aimed at evaluating the in vitro antimicrobial activity of various extracts of the stem bark of Bauhinia variegata Linn. In the current study, the petroleum ether, chloroform, ethyl acetate, ethanol and aqueous extract of stem bark of B. variegata L. was tested against standard bacterial and fungal cultures. The test was performed by agar well diffusion method on nutrient agar media and Sabouraud’s dextrose media for bacterial and fungal cultures respectively. Among the solvent extracts, ethanolic extract was effective against all the tested bacteria and fungi. Ethanolic extract exhibited maximum inhibitory activity against Klebsiella. And the least activity was observed for Staphylococus aureus. For fungi ethanolic extract was more effective against Aspergillus fumigates. Key words: Antibacterial, Antifungal, Bauhinia variegata L.