Several pharmacological activities like anti-cancer, anti-microbial, antibacterial, antifungal, and anti-viral activity have been attributed to tetrahydrocarbazole. The above observations prompted us to synthesize some novel tetrahydrocarbazole derivatives as possible anticancer agents. A series of novel tetrahydrocarbazole derivatives have been synthesized by the reaction of tetrahydrocarbazole with substituted aromatic aldehydes. The starting material, tetrahydrocarbazole were prepared by Fischer indolisation reaction of cyclohexanone with phenylhydrazine in the presence of acetic acid. The cycloaddition of tetrahydrocarcazole and substituted aromatic aldehydes gives tetrahydrocarbazole derivatives (A1-A10). The structures of synthesized derivatives were confirmed by IR, 1HNMR and Mass spectrum. The synthesized compounds were screened for their in-vitro anticancer activity. The anticancer activity data of the synthesized derivatives were found to be potent activity. Key words: Phenyl hydrazine, Cyclohexanone, Tetrahydrocarbazole derivatives, In-vitro anticancer activity.