The objective of the study was to investigate the anti-oxidant and anti-inflammatory effect of aqueous extract of Ocimum sanctum leaf. The antioxidant and anti-inflammatory activities were evaluated using in vitro Ferric reducing power, Free radical scavenging activity by DPPH  method, hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity, Hyaluronidase inhibition assay. The effect was compared with a known antioxidant agent (BHA/Ascorbic acid). It was found that the extract at different concentration exhibited significant dose dependent antioxidant and anti-inflammatory effect Key words: Ocimum sanctum, Free radical, DPPH, Hyaluronidase inhibition