The aim of the study was to prepare a poly herbal formulation containing five herbs in the base of sesame oil and comparing its activity by using the carrageenan induced rat paw oedema model. The plants used in the study were Pluchea lanceolata, Vitex negundo, Solanum xanthocarpum, Cleorodandrum phlomoides and Curcuma longa. The oil obtained was applied topically to check the anti-inflammatory activity and comparing its efficacy with the standard which was marketed topical anti-inflammatory oil. The oil having more constituent of Vitex negundo in poly herbal formulation was found out to be having more potency in comparison to rest of the prepared samples as well as the marketed sample.