The main objective of the present investigation is to evaluate the analgesic activity of methanolic extract of Nibea maculata and Johnius dussumieri on mice. Analgesic activity of methanolic extract of Nibea maculata and Johnius dussumieri at a dose of 50 mg/Kg and 100 mg /Kg were evaluated against drug pentazocine at a dose of 5 mg/Kg. Adult Swiss albino mice of either sex of six numbers in each group were under taken for study and evaluated by tail flick and tail immersion method. The both doses of Nibea maculata crude methanolic extract and Johnius dussumieri crude methanolic extract were found to produce significant (P