Medicinal plants play a vital role to preserve human health. Herbs valued for centuries as a traditional medicine, has been used to treat various human ailments. The leaves of Coleus aromaticus are useful in cephalagia, otalgia, anorexia, dyspepsia, flatulence, colic, diarrhoea, cholera, halitosis, convulsions, epilepsy, cough, asthma, hiccough, bronchitis, strangury, hepatopathy and malarial fever. Annona squamosa Linn is used as an antioxidant, antidiabetics, hepatoprotective, cytotoxicactivity, genetoxicity, antitumor activity, antilice agent. We report here the effects of various plants extracts on the cell viability. The cell viability assay revealed that the aqueous extract of Coleus aromaticus leaves and methanolic extract of Annona squamosa leaves showed significant results. Further phytochemicals screening of the extracts of both the plants revealed presence of Flavonoids on the above extracts. The total flavonoids content were determined by aluminum chloride colorimetric method. The total flavonoids contents of aqueous extract Coleus aromaticus & methanolic extracts of Annona squamosa leaves were found to be 2.60% and 2.4% respectively. Key words: Coleus aromaticus, Annona squamosa,Cell viability , Spectrophotometric.