Barlaria buxifolia Linn is one of the medicinal plants well documented traditionally in Ayurveda system of medicine and is highly valued in modern medicine owing to the presence of alkaloids, flavonoids, tannins, phenolic compounds, steroids. The plant is reported to contain phenol, flavonoid and tannin; phenolic and flavonoids compounds are reported to possess antioxidant and hence the plant may be used as organ protective. Keeping this in view, the plant was analysed for total phenol, flavonoid and tannin content. Catechol, quercetin and tannic acid reagents were used as standards for calibration of total polyphenols, flavonoids and tannins respectively. The quantification of total polyphenol, falvonoid and tannin content showed 14.65mg/gm catechol, 26.80mg/gm quercetin and 11.32mg/gm tannic acid equivalent respectively, the study indicates that the leaves of Barlaria buxifolia Linn exhibits the highest flavonoid, phenolic and tannin content. It can be used potentially as a readily accessible source of natural antioxidant. Key words: Catechol, Phenol, Flavonoid, Quercetin and Tannin, Barlaria buxifolia Linn