A rapid and sensitive High Performance liquid chromatography (HPLC) method has been developed and validated. The analyte was extracted from human plasma by simple precipitation technique. Nifedipine was used as the internal standard. A Princeton C18 column provided chromatographic separation of the analyte. A simple, selective, rapid, precise and economical reverse phase High Pressure Liquid Chromatographic method has been developed for the estimation of Lercanidipine in human Plasma. The method was carried out on a Princeton C18 (250 mm x 4.6 mm i.d., 5µ) column with a mobile phase consisting of acetonitrile: Water (adjusted to pH 3.5 using orthophosphoric acid) (55:45 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 235 nm. The retention time of Lercanidipine Nifedipine, was 5.31, 10.00 min, respectively. The proposed method has been validated with linear range of 5.0-250.0 ng/ml for Lercanidipine. The precision and accuracy values are within 10%. The overall recovery of Lercanidipine was 96.4 %. The developed and validated method was applicable for the pharmacokinetics studies.