Urolithiasis in its different forms is a frequently encountered urological disorder. For many years it has been at the forefront of urology. In the present study ethanolic extract of whole plant of Saccharum spontaneum (Linn.) was studied for its antiurolithiatic activity against most common type of renal stone i.e. calcium oxalate. Lithiasis was induced in rats by fed with a calculi-producing diet (CPD: commercial diet mixed with 3% glycolic acid) for 28 days. Glycolic acid treated rats showed significant increase (p < 0.05) the activities of oxalate synthesizing enzymes such as glycolic acid oxidase (GAO) in liver and lactate dehydrogenase (LDH) in serum, urine kidney and liver Administration of the ethanolic extract of S.spontaneum (200mg and 300/kg b.wt.dose-1 day-1oral-1) has significantly ameliorated to near normalcy in the curative group.. The results of the present study confirmed that S.spontaneum can be used as a curative agent for urolithiasis.