Treatment of myocardial infarction (MI) has undergone major advances in recent years, including reductions in mortality and hospital stays. Cardioprotective effects Ocimum gratissimum fresh leaves were evaluated in rat model having acute MI induced by Musa acuminata. Eating bananas on an empty stomach is not good to the health, because when stomach is empty, there is nearly no food in the stomach that can be digested. If this movement eats bananas, it will speed up the stomach's movement and the promotion of blood circulation, to increase the heart load. It is very easy to induce the myocardial infection. Adult male rats were divided into 4 groups. 3 groups receive Musa acuminata (banana) and 1 group Test drug. Cardio toxicity, evident from increased activities of Serum Creatine phosphokinase, Lactate Dehydrogenase, Aspartate Transaminase and Alanine Transaminase in Musa acuminata administered rats, and it was reversed by Ocimum gratissimum treatment. Musa acuminata administered rat show abnormal levels of SOD, Catalase along with high Malondialdehyde levels. The results of biochemical observation of serum and heart tissue were supplemented by histopathological examinations of rat heart sections to confirm the myocardial injury. These findings highlight the efficacy of Ocimum gratissimum as a Cytoprotectant in Musa acuminata induced cardio toxicity.