Gossypium barbadense L. is a medicinal Malvaceae used as an indispensable intrant in the endogenous medication of skin super infection caused by shingles in Côte d'Ivoire. The objective of this study was to study the analgesic and toxic effects in vivo of the extract with ethyl acetate GB2.3, obtained from the hydroalcoholic crude extract of the leaves of Gossypium barbadense L., and to characterize a phytocomponent of the extract. The results obtained showed that the extract showed a significant analgesic effect by inhibition at 73.66% of the pain induced by acetic acid in mice and that it had insignificant toxicity at the dose of 100 mg/kg body weight. A phytocompound was isolated with a yield of 15.21% by HPLC, whose molecular structure was determined by spectrometry (MS, IR, NMR 1H, 13C).