The present study was designed to investigate the cardioprotective effects of herbal formulation in rats with isoproterenol-hydrochloride (ISPH) induced myocardial infraction. Adult male Wistar Albino rats were pre-treated with herbal formulation daily for a period of 4 weeks. After the treatment period, ISPH (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISPH induced myocardial damage indicated by cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, SGOT, angiotensin converting enzyme. The activities of antioxidant enzymes such as superoxide dismutase were significantly decreased in hearts after ISPH-induced myocardial infraction. However, pre-treatment of ischemic rats with herbal formulation brought the biochemical parameters to near normalcy, indicating the protective effect of herbal formulation against ISPH-induced ischemia in rats.