Nelumbo nucifera (L.) belonging to Nelumbonaceae commonly known as lotus, aquatic herb grown and consumed throughout Asia. It is known in Sanskrit-Svetakamala, English-Sacred Lotus, Hindi- Kanwal and French-Nelumbo. All parts of N. nucifera used for various medicinal purposes in various systems of medicine including folk medicines, Ayurveda and Chinese traditional medicine. Many chemical constituents have been isolated from this plant.  However, the bioactive constituents of lotus are mainly alkaloids and flavonoids. Traditionally the whole plant of lotus was used as astringent, emollient, diuretic, diarrhea and in the treatment of tissue inflammation and seeds are used in the cancer treatment. In this research work the fixed oil was isolated from lotus seeds with a solvent extraction method and its physicochemical and phytochemical parameters were evaluated along with TLC parameter. Seeds organoleptic, microscopical & physicochemical parameters were also evaluated.