In this study, new Chitosan/4-Hydropxycoumarin films were prepared and characterized. The influence of 4-Hydroxycoumarin on the surface morphology, thermal behavior of the chitosan films were studied. The experimental studies showed that surface morphology becomes uniform and surface roughness increases with increasing the concentration of 4-Hydroxycoumarin in the chitosan film. The appreciable intermolecular interaction among the chitosan and 4-Hydroxycoumarin is confirmed by the FTIR study. The thermal properties of the chitosan films slightly increased with the increasing concentrations of 4-Hydroxycoumarin. Also, the presence of single glass transition temperature in all Chitosan/4-Hydroxycoumarin films suggests the components present in the film were miscible. Due to the existence of 4HC into the chitosan film, the crystalline nature and water contact angle of CS decreases for the C4HC films. It can be expected that, the best properties of Chitosan/4-Hydroxycoumarin films were recorded in the study may play a vital role in food packaging and coating applications.