High-resolution ultrasonic spectroscopy (HR-US) is an analytical technique based on the principle of measurement of velocity and attenuation of ultrasonic compression waves propagating through the analyzed sample. HR-US is advantageous over the other spectroscopic technique.This review directs toward the wide applications and recent advances in high-resolution ultrasonic spectroscopy. Ultrasonic velocity and ultrasonic attenuation are the key parameter in the material analysis, microscopic particle size determination in suspension, emulsion and in the analysis of other biochemical products. HR-US spectroscopy in combination with liquid chromatography system has great importance in the analysis of colloidal systems. Recently the HR-US system is used as a detector in titration technique to analyze the sample on the basis of their intermolecular interaction.