Grewia asiatica Linn. belonging to family Tiliaceae is commonly known as Phalsa found through out India. It is an important medicinal plant used in inflammation, diabetes, fever, blood disorders, cooling agent, astringent, postural eruption etc. As a herb it is used widely in the Indian traditional system, it was thought worthwhile to undertake the standardization of its aerial parts. Evaluation of the leaves were carried out to determine the effect of various drug extract on number of microbial and fungal strain. Leaves exhibited moderate to significant antibacterial and antifungal activity against all the tested bacterial strains. The chloroform extract exhibited moderate antibacterial and antifungal activity. The petroleum ether extract was devoid of any antibacterial and antifungal activity. While mentioned all the organisms are susceptible to alcoholic as well as  aqueous extracts.