A New simple, precise, accurate and rapid RP-HPLC method was proposed for determination of Dorzolamide hydrochloride from pure and its dosage form. A Symmetry Hypersil C18   (250 × 4.6mm, 5µ) column in isocratic mode with mobile phase phosphate buffer (pH 6.2): Acetonitrile (60:40) at a flow rate of 1ml/min. The effluent was monitored at 254nm. The retention time was 3.337min for Dorzolamide hydrochloride. The linearity range was found to be 20 – 120 µg/ml. The developed method was validated for parameters like specificity, accuracy, ruggedness and robustness and ascertained values were found to be within limits. The method has significant advantages in terms of shorter analysis time, selectivity and accuracy then previously reported method and indicates that the method can be considered suitable for carrying out quality control &routine determination of Dorzolamide hydrochloride in bulk and pharmaceutical dosage form.