Mosapride citrate a potent anti-histaminic drug, drug which has short half life, makes the development of sustained release (SR) forms extremely advantageous. Therefore, the present investigation of this study was to develop Mosapride citrate SR matrix tablets that provide complete drug release that starts in the stomach to rapidly alleviate the painful symptoms and continues in the intestine to maintain therapeutic effect. Mosapride citrate  showed maximum absorption at wavelength 274 nm in Acetate buffer pH 4.0, Drug-polymer compatibility studies by FTIR gave confirmation about their purity and showed no interaction between drug and selected polymers. Various formulations were developed by using release rate controlling and gel forming polymers like HPMC (K4M, K100M) by direct compression method. From among all the developed formulations, F6 formulation sustained the drug release for longer period of time as compared to other formulations. So, F6 was selected as the best formulation. It was concluded that the release followed zero order kinetics, as the correlation coefficient (R2 value) was higher for zero order release, so the drug release mechanism is controlled release. The best formulation was found to be stable during stability studies for two months. Thus, best formulation satisfied physicochemical parameters and in vitro drug release profile requirements for a sustained drug delivery.