A series of novel benzimidazoles derivatives were synthesized in convenient, easy and cheap way. These benzimidazoles are characterized by bearing amino substituents (morpholine and N-methylpiperazine) at positions 5, in addition to pyrimidine, pyridine, furyl, thienyl and pyryl with amide linkage at positions 2. The methodology of such synthetic routes was represented in synthesis of novel structures that the microbes have never been presented with before; that would hopefully prevent the process in which microbes resist antimicrobial drugs. The structures of all new compounds were identified by 1H-NMR, 13C-NMR, M.S and FT-IR spectroscopic techniques and elemental analysis. All the compounds synthesized in this work were examined for their in vitro antimicrobial activities against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), and the fungi (C.albicans and A.niger). Compared to Ciprofloxacin and Fluconazole as the reference substances, some of the synthesized compounds showed high antibacterial and antifungal activities against studied strains with inhibition zones between (12-27) mm.