To investigate the effect of Aluminium chloride on the activities of testicular enzymes in rat. Forty male Albino rats were divided into four groups (n=10). Treatment was given for a period of 45 days daily. Group-I Control given only distilled water, Group-II treated with aluminium chloride 50 mg/Kg body wt. Group-III treated with Vitamin E 20mg/Kg body weight. Group-IV treated with aluminium chloride+ Vitamin-E. The animals were sacrificed after 24 hours duration. Biochemical studies were observed in testis. Marker enzymes ALP, ACP and LDH, SDH activities were decreased significantly with control. Lipid peroxidation was increased significantly in aluminium chloride treated rats. The administration of AlCl3+Vit E shows protective effect from the toxicity.