A simple, sensitive, rapid, precise, and accurate Spectrofluorimetric method has been developed for estimation of Hydroquinone (HYQ) its pharmaceutical dosage forms.HYQ showed good fluoroscence intensity in double distilled water and so Double distilled water was selected as a solvent for estimation of HYQ. The optimized excitation and emission wavelength were 290 nm and 337 nm respectively with 2.5nm slit width for HYQ determination. The linear regression data for the calibration curves shows a good linear relationship over the concentration range of 50 to 900 ng/mL for HYQ with respect to fluorescence intensity. The LOD and LOQ values were found to be 6.88 ng/mL and 20.85 ng/mL respectively. The developed method was validated in terms of linearity, precision, accuracy, limit of detection and quantification, robustness as per International Conference on Harmonization Q2 (R1) guidelines. The utility of the developed method has been demonstrated by assay of commercially available cream formulations. The developed spectrofluorimetric method can be successfully applied for routine quality control of HYQ from its formulations.