The aim of the present study was the handling of  laboratory animals ,there sources ,routs of administrations Laboratory animals form the life-line of biomedical research and play a very vital role in the drug development programmes. There is an ever-growing need for better laboratory animal facilities for biomedical research, production of antisera, vaccines and novel drugs, especially to combat the dreadful AIDS and other diseases. The need to maintain the animals under standard animal husbandry conditions, to handle them gently and humanely, and limit unnecessary usage by strictly enforcing ethical considerations demands utmost attention. The present review includes information about housing and environmental condition, feeding techniques, safety handling for animals, routes of administration of drugs, guidelines of animal ethical committee, sources of animals, disposal of animals and also experimentation. Key words: Biomedical Research, Dreadful, AIDS, Antisera, Vaccines.