Marketed polyherbal syrup is made from five crude drug decoction, which acting with essential diuretic principles, which are Mimusops elengi, Santalum album, Crateva nurvala, Tribulus terrestris, Andropogon muricatus. The aim of this study is to evaluate diuretic activity of such marketed polyherbal formulation in wistar albino rat. Dose of marketed polyherbal syrup is 1ml/200gm, Frusemide10mg/kg. Evaluation of diuretic effect is done by measuring urine volume, sodium potassium and chloride excretion. We can conclude that marketed URIFLUX syrup produced notable diuretic effect which appeared to be comparable that produced by reference standard diuretic Frusemide.