This study describes the development and validation for the determination of Prasugrel Hydrochloride in bulk and pharmaceutical dosage form by the first-order derivative UV spectroscopy method. The quantification was achieved by the first-order derivative spectroscopy method at 248 nm over the concentration range of 5-25 μg/ml for estimation of Prasugrel Hcl (r2=0.9993) keeping methanol as solvent in zero order derivatization and same 5-25 μg/ml for estimation of Prasugrel Hcl (r2=0.9981) in methanol upon first order derivatization. Procedure does not require prior separation of components from the sample. LOD values for Prasugrel Hclwas found to be 0.591μg/mL for zero order derivatization and upon first order derivatization 0.165 μg/mL. LOQ values for Prasugrel Hclwas found to be 1.970 μg/mL for zero order derivatization and upon first order derivatization0.55 μg/mL. The results of analysis have been validated statistically and recovery studies carried out in the range 50-150% to confirm the accuracy of the proposed method. The relative standard deviation was found to be