The sustained release of Baclofen is desired because of its short biological half-life. Microspheres of Baclofen were prepared using the non-aqueous emulsification solvent evaporation method. The impacts of different factors such as type of Eudragit polymer as matrix polymer, Eudragit: HPMC K4M ratio on the characteristics of the microspheres were investigated. The morphology of microspheres was studied using optical and scanning electron microscopy and it was shown that microspheres had a spherical shape and smooth surface. The percentage yield of microspheres of all formulation was in the range of 94.01% to 99.12%. The drug content determination showed that even if the polymer composition was changed the solvent evaporation process was highly efficient to give microspheres having maximum drug loading. In termination, the prolonged sustained release time and enhanced stability resulting from the Baclofen microspheres might make contribution to its use.