The present report deals with the study of phytochemicals present in Padina tetrastromatica Hauck collected from the south east coast of Tamil Nadu, India. The phytochemicals of different extracts was analyzed by the standard procedure for UV-Vis spectroscopic and HPLC. The UV-Vis phytochemical profile of different extracts of Padina tetrastromatica Hauck was predicted. The qualitative HPLC fingerprint profile of methanol extract of Padina tetrastromatica Hauck was chosen at a wavelength of 660 nm due to sharpness of the peaks and suitable baseline. The profile showed one prominent peak at a retention time of 1.500 min and seven moderate peaks were also noted at the retention times of 2.220, 2.400, 2.500, 2.800, 3.000, 3.300 and 6.300 min respectively. The present study on Padina tetrastromatica Hauck fashioned a novel phytochemical marker in standardization of the quality of the drug used for medicine.