The main aim of this study was to evaluate the effect of Colgate Dental Cream on Catfish. Investigation was carried out using Catfish weighing about 475-525 g. Fishes were maintained for 24 h in the water, dissoved with 100 mg of Colgate Dental Cream.  Reduced serum total protein, albumin, glucose and urea, concomitant with a reduced concentration chloride ions were observed. Enzymes such as alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) enzyme activities were changed significantly. In addition, structural architecture of  liver, heart, gills, muscle and kidney were also changed. Biochemical and histopathological changes in the catfish were due to the fact that there was an increased demand for energy under stress to cope up with the detrimental effects of the dental cream.