Heterocyclic compounds have gained immense importance in human life because of their variety of applications, particularly these compounds have been successfully tested against several diseases and therefore have acquired medicinal importance. Titled Bis-Pyrazoles have been synthesized by condensation of phenyl hydrazine hydrochloride and hydrazine hydrate respectively with Bis-chalcone dibromides in pyridine medium. All compounds have been evaluated for their in vitro growth of inhibitory activity against one Gram-positive strain Staphylococcus aureus and three Gram-negative strains like Escherichia coli, Proteus mirabilis and Salmonella typhi using paper disc-method. The culture medium was nutrient agar medium. Most of the titled Bis-pyrazoles are more or less effective against these microorganisms. Hence for treatment of diseases these Bis-pyrazoles can be used by test pathogens only when they do not have any toxic and other side effects.