The present study was conducted to demonstrate the clinically efficacy of few probiotic strains freeze dried in the laboratory in acute and chronic diarrhea. The strains used in the study were isolated from commercially available probiotics and identified in the laboratory and finally subjected to freeze drying in pure cultures. After freeze drying these probiotics were filled in capsule aseptically and given to the patients with acute and chronic diarrhea to demonstrate their clinical effectiveness. This was a blind placebo control study which included healthy volunteers as well. The effectiveness of the treatment was assessed by the recovery of the ailment and improvement of the symptoms associated with the illness. Dehydrated skimmed milk was used as placebo. From the result of clinical studies it can be concluded that probiotics make a significant alternate treatment to control acute diarrhea and improve chronic diarrhea.