A series of chalcones was synthesized by the Claisen-Schmidt condensation and the structures of 1-(4-bromophenyl)-3-phenylprop-2-en-1-ones were established with the help of IR and NMR study, then their effect was observed on bovine serum albumin. We have found that the synthesized chalcones interacted with bovine serum albumin irrespective of position and nature of substituent. 1-(4-bromophenyl)-3-(3-methoxyphenyl)-prop-2-en-1-one has been found to interact with BSA maximally.