Microorganisms play a very important role in the eco-friendly and green synthesis of metal nanoparticles. The inherent, clean, nontoxic and environment friendly. The present study focuses on the rapid synthesis of titanium nanoparticles by Iraqi Lactobacillus isolates. Seventy five isolates  from(120) samples included35vaginal swabs from healthy women, 45 infant stools and 40 yoghurt were isolated and identified to species, then tested for TINPs production. Results showed that only six(8%) isolates produced titanium nanoparticles ,titanium nanoparticles were characterized with Atomic Force Microscopy (AFM) and found the average size ranges from 70.98 to 105.37  nm. The best of our study, this is the first report on synthesis of titanium nanoparticles by Iraqi Lactobacillus isolates from different sources.