Synthesis of coumarin based random copolyester was prepared by the reaction of biscoumarin monomer 1 and anthroquinone3 with azeloyl chloride through interfacial polycondensation technique by using phase transfer catalyst. The chemical structure of the copolyester was investigated by Fourier-transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR) spectroscopy. Disc diffusion method was employed to investigate the antifungal activity of the synthesized copolyester.