This paper signifies the process of aseptic germination of seeds of Taraxacum officinale, using various sterilizing agents and their influence on the physiology of seed. The three sterilizing agents, mercuric chloride (HgCl2), sodium hypochlorite (NaOCl) and ethanol (CH2OH) were used in the experiment alone and also in combination with each other in various concentrations. The degree of contamination, seed colour and % of seed germination during the course of experiment was studied. The aim of this study is to establish best surface sterilization for in-vitro propagation of Dandelion. The seeds of Taraxacum officinale were tried to germinate aseptically under various conditions and finally it was observed that seeds showed good germination using sodium hypochlorite (NaOCl) at concentration of 0.1% for 7 minutes.