The present work was defined to develop area under curve method for antipsychotic drug by UV spectrophotometric analysis which was economical, precise and an accurate method for estimation of Quetiapine fumarate. This method was based on area under curve of UV spectrum between 287 to 297 nm and validated as per ICH guideline Q2 (R1). The linearity in the range was found to be 4-14 μg/ml. The result of correlation coefficient was 0.999. The results of percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. The values of the recovery studies (99.65 % to 101.04 %) showed good accuracy of the method. LOD and LOQ were calculated as 0.3806 and 1.153μg/ml, respectively. The developed method can be applied for routine estimation of Quetiapine fumarate in bulk and tablet dosage forms.