An organism is called “susceptible” to a drug when the infection caused by it is likely to act in response to treatment with this drug, at the suggested dosage. There are many bacterial species, including E.coli are showing an increasing resistance to antibiotics and E.coli is an important pathogen of urinary tract. The growing Prevelance of antimicrobial resistance is a most important health problem and is linked with high morbidity and mortality. Antimicrobial susceptibility pattern of gram negative was observed towards Nitrofurantoin (55.38%) followed by Cotrimaxazole (22.96%), amikacin (25.93) and Ceftriaxone (12.85%) in case of E.coli and Klebsiella. Enterococcus shown maximum sensitivity to linezolid (89.11%) followed by Nitrofurantoin (51.69%), but ofloxicin (9.7%), Imipenem (9.41%) and Amikacin shown very low sensivity. So, the decision on selection of antibiotic therapy should be made in discussion with microbiologist and pharmacist.