Trees and shrubs products have been used as principal ingredients that aid human health and well-being. Based on the literature review and traditional uses of plant Annonasquamosa (Family; Annonaceae), the present study was aimed to evaluate the CNS depressant activity of ethanol, petroleum ether and aqueous extracts of the leaves of AnnonaSquamosa in albino mice. The rotarod test and diazepam induced sleep test was used to evaluate the activity of extracts interfering with motor co-ordination and sleeping time at a dose of 200 mg/kg. 30 minutes after i.p. administration of extracts. The difference in the fall off time from the rotating rod and onset and duration of sleeping time between the control and the treated mice was recorded. It can be inferred from the results that the A. squamosaleaves extracts were exhibiting interesting aimed activity against the control group, however not on par with that of standard employed. Based on the results we can conclude that the ethanol crude extract of A. squamosaleaves exhibit significant CNS depressant activity.