Barleria gibsoni belonging to the family Acanthaceae is commonly known as Neel Koranti.  Several species of Barleria are reported for treatment of inflammation associated diseases like Gout, Rheumatism and swelling etc. So, the present work has been taken for phytochemical screening and to investigate anti-inflammatory activity. Anti-inflammatory activity was done by Carrageenan induced hind paw edema method using a plethysmometer. Ibuprofen was used as a standard drug and control receives saline. The test groups received 200 mg/kg and 400 mg/kg of Ethanolic extract of Barleria gibsoni. Phytochemical screening of EEBG revealed the presence of phytocontituents like carbohydrates, tannins and flavonoids. In acute oral toxicity study it was observed that there was no mortality at any doses up to 2 mg/kg. The EEBG shows significant anti-inflammatory activity in carrageenan induced rats.