Lactic acid bacteria are a group of gram positive, non-sporing, cocci or bacilli, which produce lactic acid as the major end product in carbohydrate fermentations. Lactic acid bacteria were isolated from different diary sources . Disease causing pathogenic bacteria from clinical specimen were collected. Antimicrobial activity of the above pathogens were tested with a set of conventional antibiotics by the disk diffusion method. Antibacterial effects of the lactic acid bacteria against the above bacterial pathogens were done by agar well diffusion method. 10 pathogenic bacteria, resistant to minimum of three antibiotics  were selected for the study. Results of antibacterial activity of the lactic acid bacteria in the study revealed, a varying type of  inhibition, which was later used for comparison. The study was performed to check whether Lactic acid bacteria could be used in patients with antibiotic resistance.