Fishes adopt themselves to some extent when natural waters have high BOD, heavy metals and ozone contamination. The present work studied the effects of hypoxia, anoxia, ozone, copper sulphate and a combination of copper sulphate + ozone on the lipid and hematological profiles of  Clarias batrachus. In case of lipid profile, total cholesterol level (195.5 ± 1.5) was highest in normal condition, triglyceride (204 ± 3.162), HDL (38 ± 1.224) and VLDL (40.75 ± 1.478) in 2 hour hypoxia, and LDL (129 ± 2.236) in copper sulphate + ozone treated condition. Analysis of blood samples showed elevation Hemoglobin level (9.7 ± 0.070) was highest in 4 hour hypoxia condition, RBC count ( 3 ± .070) and glucose level (186.75 ± 1.478)  in 2 hour hypoxia , PCV percentage (28 ± 0.707) in anoxia , and total protein in normal (6 ± 0.070) and 2 hour hypoxia (6 ± 0.141) conditions.