The aim of the present work was to formulate and evaluate in situ gelling system of levofloxacin hemihydrate. Levofloxacin hemihydrate is an antibacterial agent which exhibits rapid pre-corneal elimination and poor ocular bioavailability when administered in the form of conventional ophthalmic solutions like eye drop. To overcome this, an attempt has been made to formulate pH induced in situ gelling system of levofloxacin to provide sustained release of drug. Polymeric carriers that undergo sol-to-gel transition upon change in pH. The levofloxacin hemihydrate in situ gelling system formulated by using polyox in combination with hydroxyl propyl methyl cellulose (HPMC K4M) which acted as viscosity enhancing agent. The developed formulation was stable, non-irritant and provided sustained release over 8-hour period and can be a viable alternative to conventional eye drops. The formulations were found to be non-irritating.